diff --git a/pandas/tests/frame/test_api.py b/pandas/tests/frame/test_api.py
index af61cd75c4540..f0527da0a3525 100644
--- a/pandas/tests/frame/test_api.py
+++ b/pandas/tests/frame/test_api.py
@@ -402,3 +402,22 @@ def test_inspect_getmembers(self):
         # GH38740
         df = DataFrame()
         inspect.getmembers(df)
+
+    def test_setitem_series_alignment_documentation(self):
+        # Test that Series assignment aligns by index as documented.
+        df = DataFrame({"A": [1, 2, 3]}, index=[0, 1, 2])
+        s = Series([10, 20], index=[1, 3])
+        df["B"] = s
+        expected = DataFrame({"A": [1, 2, 3], "B": [np.nan, 10, np.nan]})
+        tm.assert_frame_equal(df, expected)
+
+    def test_setitem_series_partial_alignment(self):
+        # Test Series assignment with partial index match.
+        df = DataFrame({"A": [1, 2, 3, 4]}, index=["a", "b", "c", "d"])
+        s = Series([100, 200], index=["b", "d"])
+        df["B"] = s
+        expected = DataFrame(
+            {"A": [1, 2, 3, 4], "B": [np.nan, 100, np.nan, 200]},
+            index=["a", "b", "c", "d"],
+        )
+        tm.assert_frame_equal(df, expected)