-
-
Notifications
You must be signed in to change notification settings - Fork 233
Open
Description
I have a tool:
cwlVersion: v1.2
class: CommandLineTool
label: Samtools-index-bam
doc: Samtools is a suite of programs for interacting with high-throughput sequencing data
requirements:
InlineJavascriptRequirement: {}
InitialWorkDirRequirement:
listing:
- $(inputs.bam)
hints:
- $import: samtools-docker.yml
- $import: samtools-bioconda.yml
inputs:
bam:
type: File
inputBinding:
position: 1
outputs:
indexed_bam:
type: File
secondaryFiles: .bai
outputBinding:
glob: $(inputs.bam.basename)
baseCommand: [samtools, index, -b]
The workflow:
class: Workflow
cwlVersion: v1.2
id: bwa_alignment_sort
doc: This workflow aligns the fastq files using bwa, sort and index the BAM file
label: bwa alignment workflow
requirements:
InlineJavascriptRequirement: {}
StepInputExpressionRequirement: {}
SubworkflowFeatureRequirement: {}
inputs:
bam: File
threads: int
outputs:
sorted_indexed_bam:
outputSource: bam_index/indexed_bam
type: File
secondaryFiles: .bai
steps:
bam_sort:
run: ../../tools/samtools/samtools-sort.cwl
label: Samtools-sort
in:
in_bam: bam
out_bam:
valueFrom: ${ return inputs.in_bam.nameroot.replace('Aligned.out', '') + "_sorted.bam";}
threads: threads
out: [out_sam]
bam_index:
run: ../../tools/samtools/samtools-index-bam.cwl
label: Samtools-index
in:
bam: bam_sort/out_sam
out: [indexed_bam]
I'm getting this error:
(cwltool) hydra:Variant-calling> cwltool --debug --validate samtools-sort_index.cwl
INFO /opt/miniconda3/envs/cwltool/bin/cwltool 3.1.20250715140722
INFO Resolved 'samtools-sort_index.cwl' to 'file:///Users/veraalva/Work/Developer/Python/cwl-ngs-workflows-cbb/workflows/Variant-calling/samtools-sort_index.cwl'
ERROR I'm sorry, I couldn't load this CWL file.
The error was:
Traceback (most recent call last):
File "cwltool/main.py", line 1134, in main
File "cwltool/load_tool.py", line 588, in make_tool
File "cwltool/workflow.py", line 44, in default_make_tool
File "cwltool/workflow.py", line 125, in __init__
File "cwltool/checker.py", line 185, in static_checker
File "cwltool/checker.py", line 429, in _check_all_types
File "cwltool/checker.py", line 37, in check_types
File "cwltool/checker.py", line 88, in can_assign_src_to_sink
TypeError: list object expected; got ruamel.yaml.comments.CommentedMap
Python 3.12.11
The CWL gets validated if in the workflow output the line "secondaryFiles: .bai" get's commented
Metadata
Metadata
Assignees
Labels
No labels